大规模细胞培养明智之选

Serum-free medium is a medium that can maintain cell growth and propagation in vitro for a longer period of time without the addition of serum. The serum-free medium is based on the synthetic medium, with the introduction of serum substitutes whose composition is fully or partially known, so that the medium can meet the requirements of cell culture while effectively avoiding the problems caused by the use of serum, and has been widely used in research and production, especially in cell engineering biopharmaceuticals. PrimeGene can provide a wide range of common cytokines for cell culture use.

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Long chain LR3 Insulin-like growth factor (LR3 IGF-1)

It is a key cytokine applied to cell culture as a non-motile component. It is specifically created for serum-free or low-serum cell culture for cell proliferation, life extension, and further enhancement of protein expression through anti-apoptosis and promotion of cell proliferation, and is ideal for use as a replacement for Insulin.

PrimeGene's LR3 IGF-1 cells offer the following benefits:

◆High purity: SDS-PAGE electrophoresis purity up to 98% or more, RP-HPLC purity up to 90% or more, in the industry leading level

◆High activity: Rat L6 cell line assay activity > 1.0 × 10⁵ U/mg；Human MCF-7 cells line assay activity > 6.7 × 10⁵ IU/mg

◆Low endotoxin: less than 0.01 EU/μg

◆Easy to use: no need for acid dissolution, can be used in cell culture after dissolution in neutral solution

◆Stable quality, available in stock

◆Industrial grade yield, suitable for any grade

Item No.

Name

Specification

Custom

105-03

rHuLR3 IGF-1, Media Grade

1mg/5mg/20mg

Inquiry

Insulin-like growth factor (IGF-1)

Insulin-like Growth Factor IGF-1, like LR3 IGF-1, can be added to serum-free medium to promote cell proliferation, extend cell life and facilitate expression of recombinant proteins.

PrimeGene's IGF-1 cells have the following advantages:

◆High activity: Human MCF-7 cells line assay activity > 5.0 × 10⁵ IU/mg

◆High purity: SDS-PAGE electrophoresis purity up to 98% or more, SEC-HPLC purity up to 97% or more, in the industry leading level

◆Low endotoxin: less than 0.01 EU/μg

◆Easy to use: no need for acid dissolution, can be used in cell culture after dissolution in neutral solution

◆Stable quality, available in stock

◆Industrial grade yield, suitable for any grade

Item No.

Name

Specification

Custom

Epidermal Growth Factor (EGF)

EGF (Epidermal growth factor) is a factor that can exert strong mitogenic effects during growth and proliferation and is present in various types of epidermal and epithelial tissues in vivo and in vitro, as well as in some fibroblasts during cell culture. Abnormal regulation of EGF production is known to be associated with the growth and progression of certain cancers.

Item No.

Name

Specification

Custom

Comparison of the properties of LR3 IGF-1 with Insulin and IGF-1

Insulin

IGF-1

LR3 IGF-1

Molecular Weight

~ 5.7 kDa

~ 7.6 kDa

~ 9.1 kDa

Molecular Weight

51

70

83

Molecular Structure

Isoelectric point

A chain 3.0; B chain 7.2

7.6

8.3

Receptor

Insulin Receptor (IR)

IR和IGF-1, II Receptor

IR And IGF-1 Receptor

Bioactivity

LR3 IGF-1 is more biologically active and has a longer half-life compared to IGF-1. In LR3 IGF-1 protein, arginine replaces glutamic acid in the third position of IGF-1 sequence, and the N-terminal end is 13 amino acids more than IGF-1. The small difference in amino acid sequence results in lower affinity binding of LR3 IGF-1 to IGF-BPs (BindingProteins) than IGF-1, growing the half-life and biological activity of LR3 IGF-1. The biological activity of LR3 IGF-1, when cultured in cells, is highly significantly increased compared to Insulin, IGF-1.

Applications

LR3 IGF-1 has a wide range of applications. Cells cultured with Insulin and IGF- can be cultured with LR3 IGF-1. For example, CHO, BHK, HEK293, Vero, PER.C6, MDCK, etc. can be cultured with LR3 IGF-1.

Production Capability

Provide with high quality GMP grade LR3 IGF-1 and IGF-1, kilogram level production capacity for industrial customers to solve the worries

For more detailed product information, please click

Product Advantages

Serum-free culture medium

01Be able to eliminate uncertainty and batch-to-batch variation in serum

02Avoids toxin contamination of blood products and the effect of original cytokines

In addition to the essential hormones, enzyme inhibitors, binding proteins, transferrin, trace elements, and pro-adhesive substances, the commonly added components of serum-free medium, especially Insulin, IGF-I, LR3 IGF-I and EGF, play a vital role in cell growth and proliferation.

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