Other specifications inquiry
Item No.
Product Name
Size
Amount
End User or Agent
Unit Name
Company Website
Contacts
Mobile
Email
Country/Region

Recombinant Murine Sonic Hedgehog

rMuSHH

Item No:621-19
Technical Data
    Synonyms
    SHH, HHG-1
    Accession
    Q62226
    GeneID
    20423
    Source
    Escherichia coli.
    Molecular Weight
    Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 176 amino acids.
    AA Sequence
    IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKITRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG
    Purity
    > 95 % by SDS-PAGE and HPLC analyses.
    Biological Activity
    Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine C3H10T1/2 cells is 0.5 - 1.0 μg/ml.
    Physical Appearance
    Sterile Filtered White lyophilized (freeze-dried) powder.
    Formulation
    Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
    Endotoxin
    Less than 1 EU/μg of rMuSHH as determined by LAL method.
    Reconstitution
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
    Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    - 12 months from date of receipt, -20 to -70 °C as supplied.
    - 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
    Usage
    This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
    SDS-PAGE
    Reference
    1. Currie PD, Ingham PW. 1996. Nature. 382:452-5.
    2. Herzog W, Zeng X, Lele Z, et al. 2003. Dev Biol. 254:36-49.
    3. Lewis KE, Eisen JS. 2001. Development. 128:3485-95.
    4. Scholpp S, Wolf O, Brand M, et al. 2006. Development. 133:855-64.
    5. Dassule HR, Lewis P, Bei M, et al. 2000. Development. 127:4775-85.
    6. Porter JA, Young KE, Beachy PA. 1996. Science. 274:255-9.
    Background
    Sonic hedgehog is one of three proteins in the mammalian signaling pathway family called hedgehog, the others being desert hedgehog (DHH) and indian hedgehog (IHH). As the hh homologues, SHH has been found to have the most critical roles in development. It acts as a morphogen involved in patterning many systems, including the limb and midline structures in the brain, spinal cord, the thalamus by the zona limitans intrathalamica and the teeth. There SHH undergoes auto-processing to generate a 20 kDa (residues 24 - 197) N-terminal signaling domain (SHH-N) and a 25 kDa (residues 198 - 462) C-terminal domain with no known signaling role.
Do you want to learn more about our product?
Try our product selector
Screening Products
Similar Product Recommendations
Contact Us
Service Hotline:400-821-3475
Address: Building 16, No. 3879 Dongchuan Road, Minhang District, Shanghai, 200245, P.R. China
Zip Code:200245
Phone:021-61072066
Order E-mail:order.PG@bio-techne.com
Inquiry E-mail:info.PG@bio-techne.com
COA Application
Thank you for your support and trust in PrimeGene, please enter the Lot No. and Catalog No. for inquiry.
Size:
Contact Us

Service Hotline:400-821-3475

Inquiry E-mail:order.PG@bio-techne.com

Inquiry E-mail:info.PG@bio-techne.com

Support E-mail:techsupport.PG@bio-techne.com

Dealer inquiry
BeiJing
ShangHai
ChongQing
JiangSu
ZheJiang
GuangDong
AnHui
TianJin
HeBei
ShanXi
NeiMengGu
LiaoNing
JiLin
HeiLongJiang
FuJian
JiangXi
ShanDong
HeNan
HuBei
HuNan
GuangXi
HaiNan
SiChuan
GuiZhou
YunNan
XiZang
ShanXi
GanSu
QingHai
NingXia
XinJiang
XiangGang
AoMen
TaiWan
Search Results

Delivery

Price Inquiry

Technical Consulting