- Synonyms
- Gamma-IP10, Small-inducible Cytokine B10
- Source
- Escherichia coli.
- Molecular Weight
- Approximately 8.6 kDa, a single non-glycosylated polypeptide chain containing 77 amino acids.
- AA Sequence
- VPLSRTVRCT CISISNQPVN PRSLEKLEII PASQFCPRVE IIATMKKKGE KRCLNPESKA IKNLLKAVSK EMSKRSP
- Purity
- > 97 % by SDS-PAGE and HPLC analyses.
- Biological Activity
- Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood T-lymphocytes is in a concentration range of 10-50 ng/ml.
- Physical Appearance
- Sterile Filtered White lyophilized (freeze-dried) powder.
- Formulation
- Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.
- Endotoxin
- Less than 1 EU/µg of rHuIP-10/CXCL10 as determined by LAL method.
- Reconstitution
- We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10 mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
- Stability & Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
- Usage
- This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
- SDS-PAGE
- Reference
- 1. Luster AD, Unkeless JC, Ravetch JV. 1985. Nature. 315:672-6.
2. Luster AD, Jhanwar SC, Chaganti RS, et al. 1987. Proc Natl Acad Sci U S A. 84:2868-71.
3. Booth V, Keizer DW, Kamphuis MB, et al. 2002. Biochemistry. 41:10418-25.
4. Dufour JH, Dziejman M, Liu MT, et al. 2002. J Immunol. 168:3195-204.
5. Angiolillo AL, Sgadari C, Taub DD, et al. 1995. J Exp Med. 182:155-62.
- Background
- CXCL10 also known as IP-10 is belonging to the CXC chemokine family. It is encoded by the CXCL10 gene. CXCL10 was originally identified in monocytes, endothelial cells and fibroblasts as a responsor to IFN-γ. This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3. CXCL10 has been shown to be a chemoattractant for activated T-lymphocytes and monocytes/macrophages. It also has other roles, such as promotion of T cell adhesion to endothelial cells, and inhibition of bone marrow colony formation and angiogenesis. The mouse homologue of human IP-10, Crg-2, has been cloned and shown to share approximately 67 % amino acid sequence identity with human IP-10.