小试体系蛋白定制案例

Service Items	Service Content	Cycle	Delivery Contents	Agreement Period	Actual Delivery Period	Project Results Presentation
Pilot Study Expression and Fermentation	E. coli Pilot Study Expression and Fermentation	2 Weeks	Gene Sequencing Report, Protein Expression Electrophoresis Report	6 Weeks	3 Weeks
Pilot Study Purification	Purification with Tag	1-2 Weeks	Purification Report
Formulation Lyophilization	Formulation Buffer Optimization	1-2 Weeks	Formulation Prescription
Testing Items and Requirements (Multiple Choice)	• Content	0.5 Weeks	Stock Solution or Lyophilized Pilot Study Sample + Quality Inspection report			• Protein Concentration>1mg/ml
	• Purity（SDS-PAGE）					• Purity (SDS-PAGE)>95 %
	• Endotoxin					• Endotoxin<0.1EU/ug

Customization of Pilot Study - Yeast Expression System

Service Items	Service Content	Cycle	Delivery Contents	Agreement Period	Actual Delivery Period	Project Results Presentation
Gene Synthesis	synthesis (codon optimization)	2 Weeks	Gene Sequencing Report	10 Weeks	10 Weeks
Carrier Construction	Yeast carrier construction (Tag to facilitate purification and Cleavage site between tag and the protein )	2-3 Weeks	Gene Sequencing Report
Pilot Study Expression and Fermentation	Yeast expression conditions optimization and fermentation optimization	2 Weeks	Protein Expression Electrophoresis Report
Pilot Study Purification	Purification: Secretory expression, Enzyme digestion and Tag removal	1-2 Weeks	Purification Report
Formulation Lyophilization	Preparation Condition Optimization	1 Weeks	Formulation Prescription
Testing Items and Requirements (Multiple Choice)	• Content	0.5 Weeks	Stock Solution or Lyophilized Pilot Study Sample + Quality Inspection report			• Protein Concentration>0.40 mg/ml
Testing Items and Requirements (Multiple Choice)	• Purity（SDS-PAGE）	0.5 Weeks				• Purity (SDS-PAGE)>98%

Customization of Pilot study - Baculovirus - Insect Expression System

Service Items	Service Content	Cycle	Delivery Contents	Agreement Period	Actual Delivery Period	Project Results Presentation
Gene Synthesis	Gene Synthesis	2-3 Weeks	Gene Sequencing Report	12 Weeks	12 Weeks
Carrier Construction	Baculovirus Carrier Construction (Tag to Facilitate Purification ), Bacmid preparation	2-3 Weeks	Gene Sequencing Report
Expression Lab scale and Fermentation	Transfection, Virus Amplification, Optimization of Expression Conditions	3 Weeks	Protein Expression Electrophoresis Report
Purification Lab scale	Purification: :Secretory Expression	1-2 Weeks	Purification Report
Formulation Lyophilization	Formulation Buffer Optimization	1 Weeks	Formulation Prescription
Testing Items and Requirements (Multiple Choice)	• Content	0.5 Weeks	Stock Solution or Lyophilized Pilot Study Sample + Quality Inspection report			• Protein Concentration>0.20 mg/ml
Testing Items and Requirements (Multiple Choice)	• Purity（SDS-PAGE）	0.5 Weeks				• Purity (SDS-PAGE)>90%

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