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Cat. #:GMP-104-07
Recombinant Human Keratinocyte Growth Factor-1/FGF-7 GMP
rHuKGF-1/FGF-7 GMP
Technical Parameters
AccessionP21781
GeneID2252
Source Escherichia coli.
Molecular Weight Approximately 18.9 kDa, a single, non-glycosylated polypeptide chain containing 163 amino acids.
AA Sequence CNDMTPEQMA TNVNCSSPER HTRSYDYMEG GDIRVRRLFC RTQWYLRIDK RGKVKGTQEM KNNYNIMEIR TVAVGIVAIK GVESEFYLAM NKEGKLYAKK ECNEDCNFKE LILENHYNTY ASAKWTHNGG EMFVALNQKG IPVRGKKTKK EQKTAHFLPM AIT
Purity > 98 % by SDS-PAGE and HPLC analyses.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 µm filtered solution in 20mM PB, pH 7.4, 500 mM NaCl, 0.02 % Tween-20.
Endotoxin Less than 0.01 EU/µg of rHuKGF-1/FGF-7 GMP as determined by LAL method.
Residual HCP The content of residual bacterial cell proteins is less than 0.10% of total proteins.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Usage This GMP product can be for research use or further manufacturing use.
Quality statement The manufacture and testing of this product is in compliance with ICH Q7a guidelines.