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Cat. #:672-02
Recombinant HBV Surface Antigen-preS2
rHBsAg-preS2
Technical Parameters
Source Escherichia coli.
Molecular Weight Approximately 5.7 kDa, a single non-glycosylated polypeptide chain containing 55 amino acids.
Quantity 10μg/50μg/1000μg
AA Sequence MQWNSTTFHQ ALLDPKVRGL YFPAGGSSSG TVNPVPTTAS PISSIFSRTG DPAPN
Purity >95% by SDS-PAGE and HPLC analyses.
Applications 1. Immunochromatography (capture and conjugate);
2. Preparing monoclonal or polyclonal antibodies for HBsAg-preS2;
3. ELISA.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20mM PB, pH 7.4, 50mM NaCl.
Endotoxin Less than 1 EU/μg of rHBsAg-preS2 as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Usage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.