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Cat. #:1004-01
Protein L, His
Protein L, His
Technical Parameters
AccessionD6S9W1
Source Escherichia coli.
Molecular Weight Approximately 41.5 kDa with a 6×His-tag on N-terminus, a single non-glycosylated polypeptide chain containing 372 amino acids. But it migrates with an apparent molecular mass of 46 kDa in SDS-PAGE.
AA Sequence MHHHHHHKEE TPETPETDSE EEVTIKANLI FANGSTQTAE FKGTFEKATS EAYAYADTLK KDNGEYTVDV ADKGYTLNIK FAGKEKTPEE PKEEVTIKAN LIYADGKTQT AEFKGTFEEA TAEAYRYADA LKKDNGEYTV DVADKGYTLN IKFAGKEKTP EEPKEEVTIK ANLIYADGKT QTAEFKGTFE EATAEAYRYA DLLAKENGKY TVDVADKGYT LNIKFAGKEK TPEEPKEEVT IKANLIYADG KTQTAEFKGT FAEATAEAYR YADLLAKENG KYTADLEDGG YTINIRFAGK KVDEKPEEKE QVTIKENIYF EDGTVQTATF KGTFAEATAE AYRYADLLSK EHGKYTADLE DGGYTINIRF AG
Protein per Gram Powder See Label.
Concentration for 1 unity DO at 280 nm 1.16 mg/ml
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Purity > 97 % by SDS-PAGE and SEC-HPLC analyses.
Specificity The recombinant Protein L is a genetically engineered protein containing 5 IgG-binding regions of protein L. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein L to ensure the maximum specific IgG binding. The recombinant Protein L is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein L binds to human, mouse, rat and pig IgG.
Endotoxin Level Less than 0.1 EU/μg of Protein L, His as determined by LAL method.
Formulation Lyophilized from additive free solution.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Dissolve in distilled water or saline.
BackgroundRecombinant Protein L contains 5 kappa-binding domain. Protein L has the unique ability to bind through kappa light chain interactions without interfering with the antibody’s antigen-binding site. This gives Protein L the ability to bind a wider range of Ig classes and subclasses than other antibody-binding proteins.